p300 CT Antibody, clone RW128 | EMD Millipore 05-257 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

p300 CT Antibody, clone RW128

catalog :

05-257

quantity :

200 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

RW128

The same clone is also sold as:

Abnova: MAB2384
Novus Biologicals: NB100-507, NBP2-80819
Invitrogen: MA1-16608
OriGene: TA309640
LifeSpan Biosciences: LS-C141192, LS-C141190, LS-C141191, LS-B215, LS-C141194

reactivity :

hamsters, human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

citations: 60

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; loading ...; fig 3f	Son S, Park S, Lee H, Siddiqi F, Lee J, Menzies F, et al. Leucine Signals to mTORC1 via Its Metabolite Acetyl-Coenzyme A. Cell Metab. 2019;29:192-201.e7 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 1g	Sarikhani M, Mishra S, Maity S, Kotyada C, Wolfgeher D, Gupta M, et al. SIRT2 deacetylase regulates the activity of GSK3 isoforms independent of inhibitory phosphorylation. elife. 2018;7: pubmed publisher
immunocytochemistry; human; 1:300; loading ...; fig 4a	Wang R, Cao X, Kulej K, Liu W, Ma T, MacDonald M, et al. Uncovering BRD4 hyperphosphorylation associated with cellular transformation in NUT midline carcinoma. Proc Natl Acad Sci U S A. 2017;114:E5352-E5361 pubmed publisher
chromatin immunoprecipitation; mouse; loading ...; fig 4e	Mathew R, Tatarakis A, Rudenko A, Johnson Venkatesh E, Yang Y, Murphy E, et al. A microRNA negative feedback loop downregulates vesicle transport and inhibits fear memory. elife. 2016;5: pubmed publisher
chromatin immunoprecipitation; human; loading ...; fig 3g immunoprecipitation; human; loading ...; fig 2b western blot; human; 1:500; loading ...; fig 2b	Liu L, Guan H, Li Y, Ying Z, Wu J, Zhu X, et al. Astrocyte Elevated Gene 1 Interacts with Acetyltransferase p300 and c-Jun To Promote Tumor Aggressiveness. Mol Cell Biol. 2017;37: pubmed publisher
immunocytochemistry; mouse; 1:500	Akiyama T, Xin L, Oda M, Sharov A, Amano M, Piao Y, et al. Transient bursts of Zscan4 expression are accompanied by the rapid derepression of heterochromatin in mouse embryonic stem cells. DNA Res. 2015;22:307-18 pubmed publisher
western blot; human; 1:2000; fig 5	Cho M, Park J, Choi H, Park M, Won H, Park Y, et al. DOT1L cooperates with the c-Myc-p300 complex to epigenetically derepress CDH1 transcription factors in breast cancer progression. Nat Commun. 2015;6:7821 pubmed publisher
chromatin immunoprecipitation; human; fig 5c	Lee Y, Li Y, Su C, Chiao C, Lin I, Hsu M. MAF1 represses CDKN1A through a Pol III-dependent mechanism. elife. 2015;4:e06283 pubmed publisher
immunocytochemistry; human	Wang R, You J. Mechanistic analysis of the role of bromodomain-containing protein 4 (BRD4) in BRD4-NUT oncoprotein-induced transcriptional activation. J Biol Chem. 2015;290:2744-58 pubmed publisher
chromatin immunoprecipitation; human; loading ...; fig 4b	Kim H, Park J, Won H, Lee J, Kong G. CBX7 inhibits breast tumorigenicity through DKK-1-mediated suppression of the Wnt/Î²-catenin pathway. FASEB J. 2015;29:300-13 pubmed publisher
western blot; mouse; fig 3	Meijer D, Sun Y, Liu T, Kane M, Alberta J, Adelmant G, et al. An amino terminal phosphorylation motif regulates intranuclear compartmentalization of Olig2 in neural progenitor cells. J Neurosci. 2014;34:8507-18 pubmed publisher
immunoprecipitation; human	Wang Q, Han C, Zhao R, Wani G, Zhu Q, Gong L, et al. p38 MAPK- and Akt-mediated p300 phosphorylation regulates its degradation to facilitate nucleotide excision repair. Nucleic Acids Res. 2013;41:1722-33 pubmed publisher
chromatin immunoprecipitation; rat immunoprecipitation; rat western blot; rat; fig 2	Yuan H, Reddy M, Sun G, Lanting L, Wang M, Kato M, et al. Involvement of p300/CBP and epigenetic histone acetylation in TGF-?1-mediated gene transcription in mesangial cells. Am J Physiol Renal Physiol. 2013;304:F601-13 pubmed publisher
chromatin immunoprecipitation; human western blot; human	Karius T, Schnekenburger M, Ghelfi J, Walter J, Dicato M, Diederich M. Reversible epigenetic fingerprint-mediated glutathione-S-transferase P1 gene silencing in human leukemia cell lines. Biochem Pharmacol. 2011;81:1329-42 pubmed publisher
western blot; rat	Bousiges O, Vasconcelos A, Neidl R, Cosquer B, Herbeaux K, Panteleeva I, et al. Spatial memory consolidation is associated with induction of several lysine-acetyltransferase (histone acetyltransferase) expression levels and H2B/H4 acetylation-dependent transcriptional events in the rat hippocampus. Neuropsychopharmacology. 2010;35:2521-37 pubmed publisher
chromatin immunoprecipitation; mouse	Schnekenburger M, Talaska G, Puga A. Chromium cross-links histone deacetylase 1-DNA methyltransferase 1 complexes to chromatin, inhibiting histone-remodeling marks critical for transcriptional activation. Mol Cell Biol. 2007;27:7089-101 pubmed
immunohistochemistry; human	Peng G, Chen S. Crx activates opsin transcription by recruiting HAT-containing co-activators and promoting histone acetylation. Hum Mol Genet. 2007;16:2433-52 pubmed
western blot; hamsters	Cook J, Krantz C, Routes B. Role of p300-family proteins in E1A oncogene induction of cytolytic susceptibility and tumor cell rejection. Proc Natl Acad Sci U S A. 1996;93:13985-90 pubmed
	Gillespie M, Palii C, Sánchez Taltavull D, Shannon P, Longabaugh W, Downes D, et al. Absolute Quantification of Transcription Factors Reveals Principles of Gene Regulation in Erythropoiesis. Mol Cell. 2020;78:960-974.e11 pubmed publisher
	Majumder P, Lee J, Rahmberg A, Kumar G, Mi T, Scharer C, et al. A super enhancer controls expression and chromatin architecture within the MHC class II locus. J Exp Med. 2020;217: pubmed publisher
	Wang Y, Zhong S, Schofield C, Ratcliffe P, Lu X. Nuclear entry and export of FIH are mediated by HIF1α and exportin1, respectively. J Cell Sci. 2018;131: pubmed publisher
	Guo X, Xu Y, Wang Z, Wu Y, Chen J, Wang G, et al. A Linc1405/Eomes Complex Promotes Cardiac Mesoderm Specification and Cardiogenesis. Cell Stem Cell. 2018;22:893-908.e6 pubmed publisher
	Phua T, Sng M, Tan E, Chee D, Li Y, Wee J, et al. Angiopoietin-like 4 Mediates Colonic Inflammation by Regulating Chemokine Transcript Stability via Tristetraprolin. Sci Rep. 2017;7:44351 pubmed publisher
	Zhou P, Gu F, Zhang L, Akerberg B, Ma Q, Li K, et al. Mapping cell type-specific transcriptional enhancers using high affinity, lineage-specific Ep300 bioChIP-seq. elife. 2017;6: pubmed publisher
	Sakai M, Tujimura Hayakawa T, Yagi T, Yano H, Mitsushima M, Unoki Kubota H, et al. The GCN5-CITED2-PKA signalling module controls hepatic glucose metabolism through a cAMP-induced substrate switch. Nat Commun. 2016;7:13147 pubmed publisher
	Choi S, Gearhart M, Cui Z, Bosnakovski D, Kim M, Schennum N, et al. DUX4 recruits p300/CBP through its C-terminus and induces global H3K27 acetylation changes. Nucleic Acids Res. 2016;44:5161-73 pubmed publisher
	Cazzalini O, Sommatis S, Tillhon M, Dutto I, Bachi A, Rapp A, et al. CBP and p300 acetylate PCNA to link its degradation with nucleotide excision repair synthesis. Nucleic Acids Res. 2014;42:8433-48 pubmed publisher
	Yang S, Kalkan T, Morissroe C, Marks H, Stunnenberg H, Smith A, et al. Otx2 and Oct4 drive early enhancer activation during embryonic stem cell transition from naive pluripotency. Cell Rep. 2014;7:1968-81 pubmed publisher
	Su C, Tzeng T, Cheng C, Hsu M. An H2A histone isotype regulates estrogen receptor target genes by mediating enhancer-promoter-3'-UTR interactions in breast cancer cells. Nucleic Acids Res. 2014;42:3073-88 pubmed publisher
	Sáenz Robles M, Shivalila C, Wano J, Sorrells S, Roos A, Pipas J. Two independent regions of simian virus 40 T antigen increase CBP/p300 levels, alter patterns of cellular histone acetylation, and immortalize primary cells. J Virol. 2013;87:13499-509 pubmed publisher
	Burke S, Updegraff B, Bellich R, Goff M, Lu D, Minkin S, et al. Regulation of iNOS gene transcription by IL-1? and IFN-? requires a coactivator exchange mechanism. Mol Endocrinol. 2013;27:1724-42 pubmed publisher
	Dangi Garimella S, Sahai V, Ebine K, Kumar K, Munshi H. Three-dimensional collagen I promotes gemcitabine resistance in vitro in pancreatic cancer cells through HMGA2-dependent histone acetyltransferase expression. PLoS ONE. 2013;8:e64566 pubmed publisher
	Hansen B, Dittrich Breiholz O, Kracht M, Windheim M. Regulation of NF-?B-dependent gene expression by ligand-induced endocytosis of the interleukin-1 receptor. Cell Signal. 2013;25:214-28 pubmed publisher
	Dancy B, Crump N, Peterson D, Mukherjee C, Bowers E, Ahn Y, et al. Live-cell studies of p300/CBP histone acetyltransferase activity and inhibition. Chembiochem. 2012;13:2113-21 pubmed publisher
	Tillhon M, Cazzalini O, Nardo T, Necchi D, Sommatis S, Stivala L, et al. p300/CBP acetyl transferases interact with and acetylate the nucleotide excision repair factor XPG. DNA Repair (Amst). 2012;11:844-52 pubmed publisher
	Lemieux M, Cheng Z, Zhou Q, White R, Cornell J, Kung A, et al. Inactivation of a single copy of Crebbp selectively alters pre-mRNA processing in mouse hematopoietic stem cells. PLoS ONE. 2011;6:e24153 pubmed publisher
	Chew Y, Adhikary G, Wilson G, Reece E, Eckert R. Protein kinase C (PKC) delta suppresses keratinocyte proliferation by increasing p21(Cip1) level by a KLF4 transcription factor-dependent mechanism. J Biol Chem. 2011;286:28772-82 pubmed publisher
	Senf S, Sandesara P, Reed S, Judge A. p300 Acetyltransferase activity differentially regulates the localization and activity of the FOXO homologues in skeletal muscle. Am J Physiol Cell Physiol. 2011;300:C1490-501 pubmed publisher
	Diel D, Luo S, Delhon G, Peng Y, Flores E, Rock D. A nuclear inhibitor of NF-kappaB encoded by a poxvirus. J Virol. 2011;85:264-75 pubmed publisher
	Ablack J, Pelka P, Yousef A, Turnell A, Grand R, Mymryk J. Comparison of E1A CR3-dependent transcriptional activation across six different human adenovirus subgroups. J Virol. 2010;84:12771-81 pubmed publisher
	Beguelin W, Díaz Flaqué M, Proietti C, Cayrol F, Rivas M, Tkach M, et al. Progesterone receptor induces ErbB-2 nuclear translocation to promote breast cancer growth via a novel transcriptional effect: ErbB-2 function as a coactivator of Stat3. Mol Cell Biol. 2010;30:5456-72 pubmed publisher
	Lee M, Partridge N. Parathyroid hormone activation of matrix metalloproteinase-13 transcription requires the histone acetyltransferase activity of p300 and PCAF and p300-dependent acetylation of PCAF. J Biol Chem. 2010;285:38014-22 pubmed publisher
	Franklin S, Zhang M, Chen H, Paulsson A, Mitchell Jordan S, Li Y, et al. Specialized compartments of cardiac nuclei exhibit distinct proteomic anatomy. Mol Cell Proteomics. 2011;10:M110.000703 pubmed publisher
	Reynoird N, Schwartz B, Delvecchio M, Sadoul K, Meyers D, Mukherjee C, et al. Oncogenesis by sequestration of CBP/p300 in transcriptionally inactive hyperacetylated chromatin domains. EMBO J. 2010;29:2943-52 pubmed publisher
	Aoyama T, Okamoto T, Fukiage K, Otsuka S, Furu M, Ito K, et al. Histone modifiers, YY1 and p300, regulate the expression of cartilage-specific gene, chondromodulin-I, in mesenchymal stem cells. J Biol Chem. 2010;285:29842-50 pubmed publisher
	Han L, Zhou R, Niu J, McNutt M, Wang P, Tong T. SIRT1 is regulated by a PPAR{?}-SIRT1 negative feedback loop associated with senescence. Nucleic Acids Res. 2010;38:7458-71 pubmed publisher
	Vempati R, Jayani R, Notani D, Sengupta A, Galande S, Haldar D. p300-mediated acetylation of histone H3 lysine 56 functions in DNA damage response in mammals. J Biol Chem. 2010;285:28553-64 pubmed publisher
	Nakata Y, Brignier A, Jin S, Shen Y, Rudnick S, Sugita M, et al. c-Myb, Menin, GATA-3, and MLL form a dynamic transcription complex that plays a pivotal role in human T helper type 2 cell development. Blood. 2010;116:1280-90 pubmed publisher
	Nalvarte I, Schwend T, Gustafsson J. Proteomics analysis of the estrogen receptor alpha receptosome. Mol Cell Proteomics. 2010;9:1411-22 pubmed publisher
	Gillotin S, Yap D, Lu X. Mutation at Ser392 specifically sensitizes mutant p53H175 to mdm2-mediated degradation. Cell Cycle. 2010;9:1390-8 pubmed
	Kimbrel E, Lemieux M, Xia X, Davis T, Rebel V, Kung A. Systematic in vivo structure-function analysis of p300 in hematopoiesis. Blood. 2009;114:4804-12 pubmed publisher
	Im S, Hammond L, Yousef L, Nugas Selby C, Shin D, Seo Y, et al. Sterol regulatory element binding protein 1a regulates hepatic fatty acid partitioning by activating acetyl coenzyme A carboxylase 2. Mol Cell Biol. 2009;29:4864-72 pubmed publisher
	Cha Molstad H, Saxena G, Chen J, Shalev A. Glucose-stimulated expression of Txnip is mediated by carbohydrate response element-binding protein, p300, and histone H4 acetylation in pancreatic beta cells. J Biol Chem. 2009;284:16898-905 pubmed publisher
	Burnett J, Miller Jensen K, Shah P, Arkin A, Schaffer D. Control of stochastic gene expression by host factors at the HIV promoter. PLoS Pathog. 2009;5:e1000260 pubmed publisher
	Degner S, Papoutsis A, Selmin O, Romagnolo D. Targeting of aryl hydrocarbon receptor-mediated activation of cyclooxygenase-2 expression by the indole-3-carbinol metabolite 3,3'-diindolylmethane in breast cancer cells. J Nutr. 2009;139:26-32 pubmed publisher
	Valbuena A, Blanco S, Vega F, Lazo P. The C/H3 domain of p300 is required to protect VRK1 and VRK2 from their downregulation induced by p53. PLoS ONE. 2008;3:e2649 pubmed publisher
	Ogura Y, Mochizuki K, Goda T. Induction of histone acetylation on the CRBPII gene in perinatal rat small intestine. Biochim Biophys Acta. 2007;1770:1289-96 pubmed
	Kim J, Yang C, Stallcup M. Downstream signaling mechanism of the C-terminal activation domain of transcriptional coactivator CoCoA. Nucleic Acids Res. 2006;34:2736-50 pubmed
	Pedeux R, Sengupta S, Shen J, Demidov O, Saito S, Onogi H, et al. ING2 regulates the onset of replicative senescence by induction of p300-dependent p53 acetylation. Mol Cell Biol. 2005;25:6639-48 pubmed
	von Mikecz A, Zhang S, Montminy M, Tan E, Hemmerich P. CREB-binding protein (CBP)/p300 and RNA polymerase II colocalize in transcriptionally active domains in the nucleus. J Cell Biol. 2000;150:265-73 pubmed

product information

Catalog Number :

05-257

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-p300 CT Antibody, clone RW128

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q09472

Host Name :

Mouse

Antigen :

p300 CT

Clone :

RW128

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-p300 CT Antibody, clone RW128

Cross Reactivity :

Human;Mouse;Rat

Background :

p300 (also EP300 or E1A binding protein p300) and CBP (also CREBBP or CREB binding protein) are two closely related transcriptional co-activating proteins (or coactivators). p300 and CBP interact with numerous transcription factors and act to increase the expression of their target genes. p300 and CBP have similar structures. Both contain five protein interaction domains, the nuclear receptor interaction domain (RID), the CREB and MYB interaction domain (KIX), the cysteine/histidine regions (CH1 and CH3) and the interferon response binding domain (IBiD). In addition p300 and CBP each contain a protein or histone acetyltransferase (PAT/HAT) domain and a bromodomain that binds acetylated lysines and a PHD finger motif with unknown function. p300 and CBP are thought to increase gene expression in three ways: (1) by relaxing the chromatin structure at the gene promoter through their intrinsic histone acetyltransferase (HAT) activity (2) by recruiting the basal transcriptional machinery including RNA polymerase II to the promoter (3) by acting as adaptor molecules.

ALT Names :

KAT3B

Immunogen :

GST-fusion to the C-Terminus of human p300

Specificity :

Recognizes p300 and co-immunoprecipitates E1A.

Package Size :

200 μg

Uses :

Immunoprecipitation;Western Blotting;Chromatin Immunoprecipitation (ChIP)

Storage :

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.