Anti-SMAD4 (CT) Antibody, clone EP618Y, rabbit monoclonal | EMD Millipore 04-1033 product information

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Anti-SMAD4 (CT) Antibody, clone EP618Y, rabbit monoclonal

catalog :

04-1033

quantity :

100 µL

clonality :

monoclonal

host :

rabbit

conjugate :

nonconjugated

clone name :

EP618Y

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - free floating section

more info or order :

EMD Millipore product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000; fig 3 western blot; mouse; 1:1000	Cruz A, Silva M, Caon T, Simões C. Addition of bone morphogenetic protein type 2 to ascorbate and β-glycerophosphate supplementation did not enhance osteogenic differentiation of human adipose-derived stem cells. J Appl Oral Sci. 2012;20:628-35 pubmed
immunohistochemistry - free floating section; mouse; 1:100	Tong K, Kwan K. Common partner Smad-independent canonical bone morphogenetic protein signaling in the specification process of the anterior rhombic lip during cerebellum development. Mol Cell Biol. 2013;33:1925-37 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100	Rizzardi A, Rosener N, Koopmeiners J, Isaksson Vogel R, Metzger G, Forster C, et al. Evaluation of protein biomarkers of prostate cancer aggressiveness. BMC Cancer. 2014;14:244 pubmed publisher
	Karathanasi V, Tosios K, Nikitakis N, Piperi E, Koutlas I, Trimis G, et al. TGF-β1, Smad-2/-3, Smad-1/-5/-8, and Smad-4 signaling factors are expressed in ameloblastomas, adenomatoid odontogenic tumors, and calcifying cystic odontogenic tumors: an immunohistochemical study. J Oral Pathol Med. 2013;42:415-23 pubmed publisher
	Bjerke G, Yang C, Frierson H, Paschal B, Wotton D. Activation of Akt signaling in prostate induces a TGFβ-mediated restraint on cancer progression and metastasis. Oncogene. 2014;33:3660-7 pubmed publisher
	Fernandes M, Antoine M, Hebert J. SMAD4 is essential for generating subtypes of neurons during cerebellar development. Dev Biol. 2012;365:82-90 pubmed publisher
	Park C, Skarra D, Rivera A, Arriola D, Thackray V. Constitutively active FOXO1 diminishes activin induction of Fshb transcription in immortalized gonadotropes. PLoS ONE. 2014;9:e113839 pubmed publisher

product information

Catalog Number :

04-1033

Subcategory :

Cell Signaling

Product Name :

Anti-SMAD4 (CT) Antibody, clone EP618Y, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q13485

Host Name :

Rabbit

Antigen :

SMAD4 (CT)

Clone :

EP618Y

Conjugate :

Unpurified

Isotype :

IgG

Product Description :

Anti-SMAD4 (CT) Antibody, clone EP618Y, rabbit monoclonal

Cross Reactivity :

Human;Rat;Mouse

Background :

SMAD4 is a member of the Mothers Against Dpp (MAD)-related family of proteins. SMAD4 is functionally distinct among the SMAD family, and is required for the assembly and transcriptional activation of diverse, SMAD-DNA complexes. SMAD4 appears to be not regulated by phosphorylation, but acts as a common mediator of TGF-β, activin, and bone morphogenetic protein signaling responses. SMAD4 is frequently inactivated in pancreatic, biliary and colorectal tumors.

ALT Names :

Deletion target in pancreatic carcinoma 4;MAD, mothers against decapentaplegic homolog 4 ;MAD, mothers against decapentaplegic homolog 4 (Drosophila);Mothers against DPP homolog 4;SMAD 4;SMAD family member 4;SMAD, mothers against DPP homolog 4;SMAD, mothers against DPP homolog 4 (Drosophila);deleted in pancreatic carcinoma locus 4;mothers against decapentaplegic homolog 4;mothers against decapentaplegic, Drosophila, homolog of, 4

Immunogen :

Synthetic peptide corresponding to residues in the C-terminus of human SMAD4.

Specificity :

This antibody recognizes SMAD4.

Package Size :

100 µL

Uses :

Western Blotting;Immunohistochemistry (Paraffin);Immunocytochemistry;Flow Cytometry;Immunoprecipitation

Storage :

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

more info or order :

EMD Millipore product webpage