HDAC2 full length (His-tag) human recombinant | EMD Millipore 03-228 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

protein

product name :

HDAC2 full length (His-tag) human recombinant

catalog :

03-228

quantity :

50 µg

product information

Catalog Number :

03-228

Subcategory :

Epitope Tags & General Use

Product Name :

HDAC2 full length (His-tag) human recombinant

Product Type :

Antibodies

Antigen :

HDAC2 full length (His-tag) human recombinant

Conjugate :

Purified

Product Description :

HDAC2 full length (His-tag) human recombinant

Background :

Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely involved in regulating chromatin structure during transcription. It associates with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA. This process is highly conserved from yeast to human.

Purity :

Purity ~50% by SDS-PAGE

Package Size :

50 µg

Uses :

Enzyme Assay

Storage :

Store at -80°C. The enzyme is stable on ice for 30 to 60 min, but may lose activity with dilution and/or prolonged storage on ice. It is recommended that thawing and addition of the enzyme to assay wells be done within as short a time as possible before start of the assay and that the thawed enzyme be kept on ice throughout. Best performance will be obtained by adding the chilled, undiluted enzyme directly to pre-warmed buffer and proceeding immediately to the addition of pre-warmed substrate. The remaining, unused enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. The enzyme is stable to at least 4 freeze/thaw cycles. To minimize the number of freeze/thaw cycles, aliquot the HDAC2 into separate tubes before storing at -80°C.