This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCEFLmGFP-Gli2
catalog :
37672
citations: 3
Reference
Hsiao C, Chang C, Ibrahim R, Lin I, Wang C, Wang W, et al. Gli2 modulates cell cycle re-entry through autophagy-mediated regulation of the length of primary cilia. J Cell Sci. 2018;131: pubmed publisher
Rosengren T, Larsen L, Pedersen L, Christensen S, Møller L. TSC1 and TSC2 regulate cilia length and canonical Hedgehog signaling via different mechanisms. Cell Mol Life Sci. 2018;75:2663-2680 pubmed publisher
Kim J, Kato M, Beachy P. Gli2 trafficking links Hedgehog-dependent activation of Smoothened in the primary cilium to transcriptional activation in the nucleus. Proc Natl Acad Sci U S A. 2009;106:21666-71 pubmed publisher
product information
Catalog Number :
37672
Product Name :
pCEFLmGFP-Gli2
article :
doi10.1073/pnas.0912180106
id5539
pubmed_id19996169
bacterial resistance :
Ampicillin
cloning :
backbonepCEFL
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
monomeric GFP was constructed by introducing the monomeric mutation, A206K, to EGFP.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3unknown
cloning_site_5unknown
promoterEF1a
sequencing_primer_3BGH-Rev
sequencing_primer_5EF1a-Fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneGli2
id14633
genbank_ids
mutation
nameGli2
shRNA_sequence
size
species
10090
Mus musculus
tags
locationN terminal on insert
tagmGFP
plasmid copy :
pCEFL was provided by J. Silvio Gutkind (National Institutes of Health).
resistance markers :
756
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA