pCI-neo-RL-ZEB2 200bmut | Addgene 35538 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCI-neo-RL-ZEB2 200bmut

catalog :

35538

citations: 2

Reference
Song Y, Jiang K, Su S, Wang B, Chen G. Clinical manifestations and epigenetic mechanisms of gastric mucosa associated lymphoid tissue lymphoma and long-term follow-up following Helicobacter pylori eradication. Exp Ther Med. 2018;15:553-561 pubmed publisher
Gregory P, Bert A, Paterson E, Barry S, Tsykin A, Farshid G, et al. The miR-200 family and miR-205 regulate epithelial to mesenchymal transition by targeting ZEB1 and SIP1. Nat Cell Biol. 2008;10:593-601 pubmed publisher

product information

Catalog Number :

35538

Product Name :

pCI-neo-RL-ZEB2 200bmut

article :

doi	10.1038/ncb1722
id	5145
pubmed_id	18376396

bacterial resistance :

Ampicillin

cloning :

backbone

pCI-neo-RL

backbone_mutation

RL was inserted into pcI-neo(Promega) to form the plasmid pcI-neo-Rl by Witold Filipowicz. Pillai R, Bhattacharyya S, Artus C, Zoller T, Cougot N, Basyuk E, Bertrand E, Filipowicz W (2005) Inhibition of translational initiation by let-7 microRNA in human cells. Science 309:1573-1576

backbone_origin

Witold Filipowicz

backbone_size

6380

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Luciferase

growth notes :

Mutations in the miR-200b seed regions of the ZEB2 3'UTR were generated using the QuikChange Multi site-directed mutagenesis kit. The ZEB2 3'UTR was cloned into the plasmid pCIneo-RL which was obtained from Witold Filiowicz. Pillai R, Bhattacharyya S, Artus C, Zoller T, Cougot N, Basyuk E, Bertrand E, Filipowicz W (2005) Inhibition of translational initiation by let-7 microRNA in human cells. Science 309:1573-1576 Please note that Addgene's sequencing results identified a single nucleotide deletion at bp# 2440 when compared to the full plasmid sequence provided by the depositing laboratory. Addgene's sequencing results are correct as the extra base in the sequence from the depositing laboratory was an error due to a sequencing artifact.

origin :

pi :

alt_names

HRIHFB2411, HSPC082, SIP-1, SIP1, SMADIP1, ZFHX1B

cloning

clone_method	Restriction Enzyme
cloning_site_3	ZbaI
cloning_site_5	XbaI
promoter
sequencing_primer_3	unknown
sequencing_primer_5	unknown
site_3_destroyed	1
site_5_destroyed	1

entrez_gene

aliases	HSPC082, SIP-1, SIP1, SMADIP1, ZFHX1B
gene	ZEB2
id	9839

genbank_ids

9839

mutation

6 Mutations in the miR-200b seed regions of the ZEB2 3'UTR

name

3'UTR of ZEB2 gene

shRNA_sequence

size

1264

species

9606

Homo sapiens