This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
FLAG-Pol-II
catalog :
35176
citations: 5
Reference
Lao L, Bourdeau I, Gagliardi L, He X, Shi W, Hao B, et al. ARMC5 is part of an RPB1-specific ubiquitin ligase implicated in adrenal hyperplasia. Nucleic Acids Res. 2022;: pubmed publisher
Lee H, Lee I, Kim J, Lee S, Kwak S, Suh M, et al. Phosphorylation of OGFOD1 by Cell Cycle-Dependent Kinase 7/9 Enhances the Transcriptional Activity of RNA Polymerase II in Breast Cancer Cells. Cancers (Basel). 2021;13: pubmed publisher
Ji J, Min S, Chae S, Ha G, Kim Y, Park Y, et al. De novo phosphorylation of H2AX by WSTF regulates transcription-coupled homologous recombination repair. Nucleic Acids Res. 2019;47:6299-6314 pubmed publisher
Lu L, Fan D, Hu C, Worth M, Ma Z, Jiang J. Distributive O-GlcNAcylation on the Highly Repetitive C-Terminal Domain of RNA Polymerase II. Biochemistry. 2016;55:1149-58 pubmed publisher
Rosonina E, Blencowe B. Analysis of the requirement for RNA polymerase II CTD heptapeptide repeats in pre-mRNA splicing and 3'-end cleavage. RNA. 2004;10:581-9 pubmed
product information
Catalog Number :
35176
Product Name :
FLAG-Pol-II
article :
doi10.1261/rna.5207204
id5105
pubmed_id15037767
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1(-)
backbone_mutation
backbone_originInvitrogen
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
-amanitin-resistant form of the largest subunit of pol II
origin :
37
pi :
alt_names
POLRA
POLR2A
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3BGH-Rev
sequencing_primer_5CMV-Fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesNEDHIB, POLR2, POLRA, RPB1, RPBh1, RPO2, RPOL2, RpIILS, hRPB220, hsRPB1
genePOLR2A
id5430
genbank_ids
mutationDeletion of the entire C-terminal domain (repeats 1-52)
nameRPBI
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagFLAG
resistance markers :
1173
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA