This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
E12-cTAP
catalog :
20916
citations: 2
Reference
Fernandez Garcia M, Moore C, Schulz K, Alberto O, Donague G, Harrison M, et al. Structural Features of Transcription Factors Associating with Nucleosome Binding. Mol Cell. 2019;75:921-932.e6 pubmed publisher
Yang Z, MacQuarrie K, Analau E, Tyler A, Dilworth F, Cao Y, et al. MyoD and E-protein heterodimers switch rhabdomyosarcoma cells from an arrested myoblast phase to a differentiated state. Genes Dev. 2009;23:694-707 pubmed publisher
product information
Catalog Number :
20916
Product Name :
E12-cTAP
article :
doi10.1101/gad.1765109
id2531
pubmed_id19299559
bacterial resistance :
Ampicillin
cloning :
backbonepBabe
backbone_mutation
backbone_origin
backbone_size4500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
The depositor noted that the E12 cTAP A218T mutation found in Addgene's quality control sequence does NOT affect plasmid function.
origin :
37
pi :
alt_names
E2A (E12 isoform)
Tcfe2a
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5BamHI
promoter
sequencing_primer_3GGACTTTCCACACCCTAACTGACAC
sequencing_primer_5GATCCTCCCTTTATCCAGCCCTC
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesA1, ALF2, E12, E12/E47, E2A, E47, KA1, ME2, Pan1, Pan2, TCF-3, Tcfe2a, VDIR, bHLHb21
geneTcf3
id21423
genbank_ids
NM_0115483
mutationTAP tag is fused to the 3' end of the E12 gene. Mutation A218T (please see depositor's comment below)
nameE12 cTAP
shRNA_sequence
size2547
species
10090
Mus musculus
tags
locationN terminal on insert
tagFlag
resistance markers :
520
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA