This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pWPT-PDX1
catalog :
12256
citations: 5
Reference
Doubleday P, Fornelli L, Ntai I, Kelleher N. Oncogenic KRAS creates an aspartate metabolism signature in colorectal cancer cells. FEBS J. 2021;288:6683-6699 pubmed publisher
Rodprasert W, Nantavisai S, Pathanachai K, Pavasant P, Osathanon T, Sawangmake C. Tailored generation of insulin producing cells from canine mesenchymal stem cells derived from bone marrow and adipose tissue. Sci Rep. 2021;11:12409 pubmed publisher
Sawangmake C, Rodprasert W, Osathanon T, Pavasant P. Integrative protocols for an in vitro generation of pancreatic progenitors from human dental pulp stem cells. Biochem Biophys Res Commun. 2020;530:222-229 pubmed publisher
Van Pham P, Thi My Nguyen P, Thai Quynh Nguyen A, Minh Pham V, Nguyen Tu Bui A, Thi Tung Dang L, et al. Improved differentiation of umbilical cord blood-derived mesenchymal stem cells into insulin-producing cells by PDX-1 mRNA transfection. Differentiation. 2014;87:200-8 pubmed publisher
Ritz Laser B, Gauthier B, Estreicher A, Mamin A, Brun T, Ris F, et al. Ectopic expression of the beta-cell specific transcription factor Pdx1 inhibits glucagon gene transcription. Diabetologia. 2003;46:810-21 pubmed
product information
Catalog Number :
12256
Product Name :
pWPT-PDX1
article :
doi10.1007/s00125-003-1115-7
id839
pubmed_id12783165
bacterial resistance :
Ampicillin
cloning :
backbonepWPT
backbone_mutation
backbone_origin
backbone_size9560
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
Cre/Lox
growth notes :
Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.
growth temp :
Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3See map
cloning_site_5See map
promoter
sequencing_primer_3
sequencing_primer_5pBluescriptKS primer
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGSF, IDX-1, IPF1, IUF1, MODY4, PAGEN1, PDX-1, STF-1
genePDX1
id3651
genbank_ids
mutation
namePdx1
shRNA_sequence
size
species
9606
Homo sapiens
tags
plasmid copy :
Human PDX1 cDNA was provided by V. Schwitzgebel.
resistance markers :
532
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA