This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
p416 25Q GPD
catalog :
1177
citations: 5
Reference
Ohkuni K, Pasupala N, Peek J, Holloway G, Sclar G, Levy Myers R, et al. SUMO-Targeted Ubiquitin Ligases (STUbLs) Reduce the Toxicity and Abnormal Transcriptional Activity Associated With a Mutant, Aggregation-Prone Fragment of Huntingtin. Front Genet. 2018;9:379 pubmed publisher
Marquez Lona E, Torres Machorro A, Gonzales F, Pillus L, Patrick G. Phosphorylation of the 19S regulatory particle ATPase subunit, Rpt6, modifies susceptibility to proteotoxic stress and protein aggregation. PLoS ONE. 2017;12:e0179893 pubmed publisher
Xu Z, Lv X, Dai Q, Lu M, Jin Z. Exogenous BDNF Increases Mitochondrial pCREB and Alleviates Neuronal Metabolic Defects Following Mechanical Injury in a MPTP-Dependent Way. Mol Neurobiol. 2018;55:3499-3512 pubmed publisher
Li L, Liu H, Dong P, Li D, Legant W, Grimm J, et al. Real-time imaging of Huntingtin aggregates diverting target search and gene transcription. elife. 2016;5: pubmed publisher
Krobitsch S, Lindquist S. Aggregation of huntingtin in yeast varies with the length of the polyglutamine expansion and the expression of chaperone proteins. Proc Natl Acad Sci U S A. 2000;97:1589-94 pubmed
product information
Catalog Number :
1177
Product Name :
p416 25Q GPD
article :
doi10.1073/pnas.97.4.1589
id29
pubmed_id10677504
bacterial resistance :
Ampicillin
cloning :
backbonep416 GPD
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
N terminal region of Ht with polyQ repeat length of 25 fused to GFP.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5XhoI
promoter
sequencing_primer_3EGFP-N
sequencing_primer_5M13R
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHD, IT15, LOMARS
geneHTT
id3064
genbank_ids
NM_002111
mutation
namehtt 25Q
shRNA_sequence
size250
species
9606
Homo sapiens
tags
locationC terminal on insert
tagGFP
resistance markers :
293
tags :
Low Copy
terms :
URA3
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA