This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.1his p66shc S36A
catalog :
10973
citations: 1
Reference
Nemoto S, Finkel T. Redox regulation of forkhead proteins through a p66shc-dependent signaling pathway. Science. 2002;295:2450-2 pubmed
product information
Catalog Number :
10973
Product Name :
pcDNA3.1his p66shc S36A
article :
doi10.1126/science.1069004
id437
pubmed_id11884717
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1 His
backbone_mutation
backbone_originInvitrogen
backbone_size5500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please note that this plasmid contains additional mutations (D2N and S38P) in the p66/SHC sequence when compared to GenBank entries NP_892113.4 and NP_001123512.1. This is the original plasmid as used in the associated publication listed below.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5EcoRI
promoter
sequencing_primer_3BGHrev
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesSHC, SHCA
geneSHC1
id6464
genbank_ids
mutationS36A
namep66shc
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHis
resistance markers :
146
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA