rabbit polyclonal
reactivity: human, rat
application: WB, ELISA, IHC
publications citing this reagent: 3

quantity: 100 ug
price:
to the supplier

rabbit polyclonal
reactivity: human, rat
application: WB, ELISA, IHC, FC
publications citing this reagent: 3

Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA3 ATPase (PA1-916) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Immunohistochemistry was performed on normal biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA3 ATPase (PA1-916) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
quantity: 100 µg
price: 299.00 USD
to the supplier

rabbit polyclonal
reactivity: human, rat, mouse
application: WB, ELISA, IC, IP
publications citing this reagent: 1

quantity: 200 µg/ml
price: 279 USD
to the supplier

goat polyclonal
reactivity: human, rat, mouse
application: WB, ELISA, IC

quantity: 200 µg/ml
price: 279 USD
to the supplier

goat polyclonal
reactivity: human, rat, mouse
application: WB, ELISA, IC, IP

quantity: 200 µg/ml
price: 279 USD
to the supplier