rabbit polyclonal
reactivity: human, rat, mouse
application: WB, ELISA, IHC

quantity: 0.1 mg
price: 245.00 USD
to the supplier

rabbit polyclonal
reactivity: human, rat, mouse
application: WB, ELISA, IHC

Immunohistochemistry of paraffin-embedded human kidney using 18295-1-AP(HRAS-Specific Antibody) at Dilution 1:50 (under 40x lens)

PC-3 cells were subjected to SDS PAGE followed by western blot with 18295-1-AP(HRAS-Specific Antibody) at dilution of 1:500
quantity: 150 ul
price:
to the supplier

mouse monoclonal
reactivity: human, mouse
application: WB, ELISA, IHC, IC, IP, FACS

Immunofluorescent analysis of Ras in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Ras monoclonal antibody (Product # MA1-012) at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Ras staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

Immunohistochemistry was performed on cancer biopsies of deparaffinized Human lung adenocarcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a Ras monoclonal antibody (Product # MA1-012) at a dilution of 1:200 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Western blot analysis of Ras was performed by loading 50ug of various whole cell lysate onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing Ras (Product # MA1-012) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product #31430) at a dilution of 1:20000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Pierce Super Signal West Pico(Product #34077).
quantity: 100 µg
price: 299.00 USD
to the supplier

mouse monoclonal
reactivity: human, mouse
application: WB, ELISA, IHC, IC, IP, FACS

Immunofluorescent analysis of Ras in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Ras monoclonal antibody (Product # MA1-012) at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Ras staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

Immunohistochemistry was performed on cancer biopsies of deparaffinized Human colon carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a Ras monoclonal antibody (Product # MA1-012) at a dilution of 1:200 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Western blot analysis of Ras was performed by loading 50ug of various whole cell lysate onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing Ras (Product # MA1-012) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product #31430) at a dilution of 1:20000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Pierce Super Signal West Pico(Product #34077).
quantity: 20 µg
price: 79.00 USD
to the supplier

chicken polyclonal
reactivity: rat, mouse
application: WB

quantity: 50 µg
price: 417.00 USD
to the supplier

rat monoclonal
reactivity: human, rat, mouse
application: WB, IHC, IC, IP

quantity: 50 ug
price: 269 USD
to the supplier

rabbit monoclonal
reactivity: human, rat, mouse
application: WB, IC, IP

quantity: 100 ul
price: 289 USD
to the supplier

rabbit polyclonal
reactivity: human, rat, mouse

quantity: 100 ug
price: 312 USD
to the supplier

rabbit polyclonal
reactivity: human, rat, mouse

quantity: 100 ug
price: 296 USD
to the supplier

rabbit polyclonal
reactivity: chicken, human, rat, mouse

quantity: 100 ug,200 ug
price: 240 USD
to the supplier

mouse monoclonal
reactivity: human, rat, dog, mouse

quantity: 100 ul
price: 325 USD
to the supplier

mouse monoclonal
reactivity: human, rat, dog, mouse

quantity: 100 ul
price: 379 USD
to the supplier

mouse monoclonal
reactivity: human, rat, dog, mouse

quantity: 100 ul
price: 325 USD
to the supplier

rat monoclonal
reactivity: human, rat, mouse
application: WB, ELISA, IC, IP, FC
publications citing this reagent: 57

quantity: 200 µg/ml
price: 279 USD
to the supplier

rabbit polyclonal
reactivity: human, rat, mouse
application: WB, ELISA, IC, IP
publications citing this reagent: 52

quantity: 200 µg/ml
price: 279 USD
to the supplier

mouse monoclonal
reactivity: human, rat, mouse
application: WB, IHC, IC, IP, IHC-P
publications citing this reagent: 30

quantity: 100 µg/ml
price: 279 USD
to the supplier

mouse monoclonal
reactivity: human, mouse
application: WB, ELISA

quantity:
price:
to the supplier

rat monoclonal
reactivity: human, rat, mouse
application: WB, IHC, IC, IP

quantity: 50 ug
price:
to the supplier

rabbit monoclonal
reactivity: human, rat, mouse
application: WB, IP, FC
publications citing this reagent: 2

quantity: 100 ul
price:
to the supplier

rabbit monoclonal
reactivity: human, rat, mouse
application: WB, IC, IP
publications citing this reagent: 2

quantity: 100 ul
price:
to the supplier

mouse monoclonal
reactivity: human, dog, mouse
application: WB, IHC, IC

quantity: 100 ul
price:
to the supplier

mouse monoclonal
reactivity: human, rat, mouse
application: WB, ELISA, IHC, IC, IP, FC

quantity: 100 ug
price:
to the supplier

rabbit polyclonal
reactivity: chicken, human, mouse
application: WB

quantity: 100 ug
price:
to the supplier

rabbit monoclonal
reactivity: human, mouse
application: WB

quantity: 100 uL
price:
to the supplier