mouse polyclonal
reactivity: human
application: WB, ELISA

quantity: 50 uL
price: quote
to the supplier

mouse monoclonal
reactivity: human
application: ELISA, WB

quantity: 100 ug
price: quote
to the supplier

rabbit polyclonal
reactivity: human
application: WB, ELISA, IP

quantity: 100 ug
price: quote
to the supplier

rabbit polyclonal
reactivity: human
application: WB, ELISA, IP
publications citing this reagent: 1

Most modifiers mature by proteolytic processing from inactive precursors (“a” = amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thioesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein)/CBC(cullin-2/elonginB/elonginC)-like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. (From Jentsch & Pyrowolakis (2000); see references below.)

Most modifiers mature by proteolytic processing from inactive precursors (“a” = amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thioesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein)/CBC(cullin-2/elonginB/elonginC)-like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. (From Jentsch & Pyrowolakis (2000); see references below.)
quantity: 100 ug
price: 289.00 USD
to the supplier

rabbit polyclonal
reactivity: human
application: WB, ELISA, IP

Most modifiers mature by proteolytic processing from inactive precursors (“a” = amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thioesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein)/CBC(cullin-2/elonginB/elonginC)-like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. (From Jentsch & Pyrowolakis (2000); see references below.)

Most modifiers mature by proteolytic processing from inactive precursors (“a” = amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thioesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein)/CBC(cullin-2/elonginB/elonginC)-like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. (From Jentsch & Pyrowolakis (2000); see references below.)
quantity: 25 uL
price: 89.00 USD
to the supplier

rabbit polyclonal
reactivity: human
application: WB, IC

Western blot analysis of Jurkat Whole Cell Lysate using PA5-19675, UBC6e primary antibody at a dilution of 1:250. Blot treated with a secondary IR Dye680-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:10000.

Immunofluorescent staining of HeLa cells using PA5-19675, anti-UBC6e antibody. The cells were fixed with PFA (4%) for 10 minutes, permabilised with BSA(1%), normal goat serum (10%) and glycine (0.3 M) in 0.1% T-BST for 20 minutes and exposed to the primary antibody at a concentration of 5 ug/ml for 1 hour at room temp. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain was DAPI (blue).
quantity: 200 ul
price: 299.00 USD
to the supplier

mouse monoclonal
reactivity: human, mouse
application: WB, IHC, FC

quantity: 100 ul
price: 325 USD
to the supplier

mouse monoclonal
reactivity: human, mouse
application: WB

quantity: 100 ul
price: 325 USD
to the supplier

mouse monoclonal
reactivity: human, mouse
application: WB

quantity: 100 ul
price: 325 USD
to the supplier

mouse monoclonal
reactivity: human, rat, mouse
application: WB, ELISA, IP

quantity: 100 µg/ml
price: 295 USD
to the supplier

goat polyclonal
reactivity: human, rat, mouse
application: WB, ELISA, IC

quantity: 200 µg/ml
price: 279 USD
to the supplier

goat polyclonal
reactivity: human, mouse
application: WB, ELISA, IC

quantity: 200 µg/ml
price: 279 USD
to the supplier

rabbit polyclonal
reactivity: human
application: WB, IHC

quantity:
price:
to the supplier

rabbit polyclonal
reactivity: human
application: WB, ELISA, IP

quantity:
price:
to the supplier

rabbit polyclonal
reactivity: human
application: WB, ELISA

quantity:
price:
to the supplier