rabbit polyclonal
reactivity: human

quantity: 100 ug
price: quote
to the supplier

rabbit polyclonal
reactivity: human
modification: phosphorylated
application: WB, ELISA, IHC, IP

Rockland's affinity purified anti-Monophosphorylated RLC Smooth and Non-Muscle Myosin pS19/20 antibody was used at 2.5 ?g/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows strong staining of both vascular and myometrial smooth muscle cells of the uterus. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.

Affinity Purified Phospho specific antibody to Monophosphorylated Regulatory Light Chain of Smooth and Non-muscle Myosin at pS19/pS20 was used at a 1:5000 dilution to detect myosin light chain by Western blot. Either 13 or 20 ul of a mouse cardiac myocyte lysate was loaded on a 4-20% Criterion gel for SDS-PAGE.  Samples were either mock-treated or CLA-treated, as indicated.  After washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG (611-103-122) preceded color development using Amersham's substrate system. Other detection methods will yield similar results. Data courtesy of the Alliance for Cellular Signaling (http://www.signaling-gateway.org).

Affinity purified phosphospecific antibody to phosphorylated regulatory light chain of smooth and non-muscle Myosin at pS19/pS20 was used at a 1:1000 dilution to detect myosin light chain by Western blot on 3T3 cell lysates. A standard urea/glycerol gel without SDS was used to separate phospho forms of regulatory light chain according to mass to charge ratios. In Panel A, reactivity of Rockland's phosphospecific antibody is shown. In Panel B, reactivity of commercially available pan reactive antibody that detects both unphosphorylated and phosphorylated forms of regulatory light chain is shown. Rockland's phosphospecific antibody detects both monophosphorylated (pSer20 Mono-P-RLC) and diphosphorylated (pThr19-pSer20 Di-P-RLC) regulatory light chain. Personal communication. J. Stull. UT Southwestern Medical Center.
quantity: 100 ug
price: 329.00 USD
to the supplier

rabbit polyclonal
reactivity: human
modification: phosphorylated
application: WB, ELISA, IHC, IP

Rockland's affinity purified anti-Monophosphorylated RLC Smooth and Non-Muscle Myosin pS19/20 antibody was used at 2.5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows strong staining of both vascular and myometrial smooth muscle cells of the uterus. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.

Affinity Purified Phospho specific antibody to Monophosphorylated Regulatory Light Chain of Smooth and Non-muscle Myosin at pS19/pS20 was used at a 1:5000 dilution to detect myosin light chain by Western blot. Either 13 or 20 ul of a mouse cardiac myocyte lysate was loaded on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock-treated or CLA-treated, as indicated. After washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG (611-103-122) preceded color development using Amersham's substrate system. Other detection methods will yield similar results. Data courtesy of the Alliance for Cellular Signaling (http://www.signaling-gateway.org).
quantity: 25 uL
price: 89.00 USD
to the supplier

rabbit monoclonal
reactivity: human
application: WB, IC, FC, FACS

quantity: 100 ul
price: 289 USD
to the supplier

goat polyclonal
reactivity: human, rat, mouse
modification: phosphorylated
application: WB, ELISA, IHC, IC, IP
publications citing this reagent: 11

quantity: 200 µg/ml
price: 279 USD
to the supplier

rabbit polyclonal
reactivity: human, rat, mouse
application: WB, ELISA, IHC, IC, IP, IHC-P
publications citing this reagent: 6

quantity: 200 µg/ml
price: 279 USD
to the supplier

mouse monoclonal
reactivity: human, rat, mouse
application: WB, ELISA, IHC, IC, IP, IHC-P
publications citing this reagent: 2

quantity: 200 µg/ml
price: 279 USD
to the supplier

rabbit polyclonal
reactivity: human
application: WB, IC, IP

quantity: 100 ul
price:
to the supplier

rabbit monoclonal
reactivity: human
application: WB, IC, FC

quantity: 100 ul
price:
to the supplier

rabbit monoclonal
reactivity: human, rat, mouse
modification: phosphorylated
application: WB

quantity: 100 ul
price:
to the supplier