mouse monoclonal
reactivity: human, mouse
modification: phosphorylated
application: WB
publications citing this reagent: 3

quantity: 100 ul
price: 274 USD
to the supplier

mouse monoclonal
reactivity: human, mouse
modification: phosphorylated
application: WB
publications citing this reagent: 3

quantity: 300 ul
price: 658 USD
to the supplier

rabbit monoclonal
reactivity: human, mouse
application: WB, IP
publications citing this reagent: 2

quantity: 100 ul
price: 235 USD
to the supplier

mouse monoclonal
reactivity: human, mouse
modification: phosphorylated
application: WB

quantity: 25 μg
price: 125 USD
to the supplier

mouse monoclonal
reactivity: human, mouse
modification: phosphorylated
application: WB

quantity: 100 μg
price: 275 USD
to the supplier

mouse monoclonal
reactivity: human, rat, mouse
application: WB, IC, Neut

quantity: 25 μg
price: 125 USD
to the supplier

rabbit polyclonal
reactivity: human
application: WB
publications citing this reagent: 3

quantity: 0.1 ml
price: 338 USD
to the supplier

rabbit polyclonal
reactivity: human
application: WB, IHC
publications citing this reagent: 3

quantity: 0.1 ml
price: 338 USD
to the supplier

rabbit polyclonal
reactivity: human
application: WB

quantity: 0.1 ml
price: 338 USD
to the supplier

mouse monoclonal
reactivity: human
application: WB

quantity: 0.1 mg
price: 345 USD
to the supplier

rabbit polyclonal
reactivity: human
application: WB, IHC

quantity: 0.1 ml
price: 440 USD
to the supplier

rabbit polyclonal
reactivity: human, rat, mouse
application: WB

quantity: 0.1 mg
price: 250 USD
to the supplier

rabbit polyclonal
reactivity: human, mouse
application: WB, IC

quantity: 100 ug
price: 296 USD
to the supplier

rabbit polyclonal
reactivity: human, mouse
modification: phosphorylated
application: WB, ELISA, IHC

quantity: 100 ug,200 ug
price: 240 USD
to the supplier

rabbit polyclonal
reactivity: human, rat, mouse
modification: phosphorylated

quantity: 100 ug
price: 296 USD
to the supplier

mouse monoclonal
reactivity: human, rat, mouse
application: WB, IHC, IP
publications citing this reagent: 2

quantity: 100 ug
price: 289 USD
to the supplier

mouse monoclonal
reactivity: human
modification: phosphorylated
application: WB

quantity: 100 ul
price: 299 USD
to the supplier

mouse monoclonal
reactivity: human, mouse
modification: phosphorylated
application: WB, IC, IP
publications citing this reagent: 1

Immunofluorescent analysis of ATM phosphorylated on pSer1981 (green) in HeLa cells left untreated (left panels) or treated with 10uM Camptothecin for 4 hours (right panels). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a phospho-ATM (pSer1981) monoclonal antibody (Product # MA1-2020), at a dilution of 1:1000 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) (top panels). A merged image with nuclei staining (blue) using Hoechst 33342 dye (Product # 62249) is shown on bottom panels. Images were taken on a Thermo Scientific ArrayScan at 20X magnification.

Western blot analysis of phospho-ATM (pSer1981) was performed using phospho-ATM (pSer1981) monoclonal antibody (Product # MA1-2020) on gamma-irradiated HeLa cells.
quantity: 200 µg
price: 239.00 USD
to the supplier

mouse monoclonal
reactivity: human
modification: phosphorylated
application: WB, IC

Immunofluorescent analysis of Phospho-ATM (green) in HeLa cells either left untreated (left panel) or treated with 50uM etoposide for 3 hours (right panel). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a Phospho-ATM monoclonal antibody (Product # MA5-15185) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or a ToxInsight Instrument at 20X magnification.

Immunofluorescence staining of Phospho-ATM in A549 cells. Cells were treated with vehicle (0.1% DMSO in media) or with 50 µM etoposide for 3 hours

Activation of ATM and p53: ATM kinase is auto-phosphorylated in response to DNA damage and is involved in DNA damage induced activation of p53 and cell cycle arrest. This is a multiplex kit, enabling the user to simultaneously measure both phospho-ATM and p53. A549 cells were treated with vehicle (DMSO) or 50 µM etoposide for 24 hrs. After staining cells with Cellomics phospho-ATM/p53 duplex activation kit, images were acquired and analyzed. Z-factor for phospho-ATM = 0.46 ± 0.11 and %COV = 11 ± 3. Z-factor for p53 = 0.44 ± 0.03 and %COV = 13 ± 1. EC50 of etoposide treatment for ATM activation is 19 µM and EC50 for p53 activation is 36 µM. Etoposide is a topoisomerase II inhibitor resulting in DNA strand break
quantity: 100 µl
price: 288.00 USD
to the supplier

mouse monoclonal
reactivity: human, rat, mouse
application: WB, IC

Western Blot analysis of ATM-kinase, using MA1-46069. Sample: Irradiated or peroxidated human fibroblasts.

quantity: 100 µl
price: 348.00 USD
to the supplier

rabbit polyclonal
reactivity: human, mouse
application: WB, ELISA

quantity: 0.1 mg
price: 220.00 USD
to the supplier

mouse monoclonal
reactivity: human, rat, mouse
conjugate: HRP
modification: phosphorylated
application: WB, ELISA, IHC

Rockland's anti-ATM pS1981 mouse monoclonal antibody (Catalog # 200-301-400) detects ATM phosphorylated on Ser 1981 by Indirect immunofluorescence microscopy. Shown are hTCEpi cells (courtesy of Dr. Danielle Robertson) infected with HSV-1 at MOI 5.0 and fixed at 8 hpi with 3% paraformaldehyde/2% sucrose for 10 min. After rinsing, cells were permeabilized with 0.5% Triton X-100 for 5 min, blocked with 3% BSA for 30 min, and stained with Rockland's primary anti-ATM pS1981 antibody overnight at 5 ug/mL (1:200). Secondary staining was performed with Alexa Fluor 594 anti-mouse antibody. Images were taken with Olympus AX70 compound epifluorescence microscope equipped with Spot RT Slider camera. Experiment was performed by Oleg Alekseev in the laboratory of Dr. Jane Azizkhan-Clifford at Drexel University College of Medicine.

Anti ATM Mab with human derived HEK293 cells treated with doxorubicin using Rockland's Protein A Purified Mab anti-ATM Protein Kinase pS1981(clone 10H11.E12). A 370 kDa band corresponding to phosphorylated ATM is detected (arrowhead, lane 2). The lysate was prepared with HALT phosphatase inhibitor (Pierce). Pre-incubation of peptide with 50 ug of immunizing phospho peptide negates specific staining (lane 1). Approximately 30 ug of lysate was added to each lane of an SDS-PAGE gel under non-reducing conditions. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody diluted 1:500 overnight at 4°C followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Mouse IgG [H&L] (code 610-132-121) for 40 min at room temperature. LICOR's Odyssey® Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.

IAnti ATM Antibody showing overlay of anti-ATM pS1981 staining. Cells were fixed 15 min after 5 Gy (IR+) of ??irradiation, then labeled with antibody. See Kitagawa et al. for additional details.
quantity: 100 ug
price: 405.00 USD
to the supplier

mouse monoclonal
reactivity: human, rat, mouse
modification: phosphorylated
application: WB, ELISA, IHC

Rockland's anti-ATM pS1981 mouse monoclonal antibody (Catalog # 200-301-400) detects ATM phosphorylated on Ser 1981 by Indirect immunofluorescence microscopy. Shown are hTCEpi cells (courtesy of Dr. Danielle Robertson) infected with HSV-1 at MOI 5.0 and fixed at 8 hpi with 3% paraformaldehyde/2% sucrose for 10 min. After rinsing, cells were permeabilized with 0.5% Triton X-100 for 5 min, blocked with 3% BSA for 30 min, and stained with Rockland's primary anti-ATM pS1981 antibody overnight at 5 ug/mL (1:200). Secondary staining was performed with Alexa Fluor 594 anti-mouse antibody. Images were taken with Olympus AX70 compound epifluorescence microscope equipped with Spot RT Slider camera. Experiment was performed by Oleg Alekseev in the laboratory of Dr. Jane Azizkhan-Clifford at Drexel University College of Medicine.

Anti ATM Mab with human derived HEK293 cells treated with doxorubicin using Rockland's Protein A Purified Mab anti-ATM Protein Kinase pS1981(clone 10H11.E12). A 370 kDa band corresponding to phosphorylated ATM is detected (arrowhead, lane 2). The lysate was prepared with HALT phosphatase inhibitor (Pierce). Pre-incubation of peptide with 50 ug of immunizing phospho peptide negates specific staining (lane 1). Approximately 30 ug of lysate was added to each lane of an SDS-PAGE gel under non-reducing conditions. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody diluted 1:500 overnight at 4°C followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Mouse IgG [H&L] (code 610-132-121) for 40 min at room temperature. LICOR's Odyssey® Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.

Anti ATM Antibody showing overlay of anti-ATM pS1981 staining. Cells were fixed 15 min after 5 Gy (IR+) of ??irradiation, then labeled with antibody. See Kitagawa et al. for additional details.
quantity: 100 ug
price: 405.00 USD
to the supplier

sheep polyclonal
reactivity: human
modification: phosphorylated
application: WB, ELISA, IP

quantity: 25 uL
price: 89.00 USD
to the supplier

rabbit polyclonal
reactivity: human
modification: phosphorylated
application: WB
publications citing this reagent: 2

quantity: 100 ug
price: 285 USD
to the supplier

mouse monoclonal
reactivity: human
application: IC

quantity: 100 ug
price: 240 USD
to the supplier

sheep polyclonal
reactivity: human
application: WB, IHC

quantity: 100 ug
price: 355 USD
to the supplier

mouse monoclonal
reactivity: human, mouse
modification: phosphorylated
application: WB, IC, IP
publications citing this reagent: 1

quantity: 200 ug
price:
to the supplier

rabbit polyclonal
reactivity: human, rat, mouse
application: WB, IP
publications citing this reagent: 1

quantity: 100 ug
price:
to the supplier

mouse monoclonal
reactivity: human
modification: phosphorylated
application: WB, IC, IP

quantity: 100 ug
price:
to the supplier

mouse monoclonal
reactivity: human, rat, mouse
application: WB, IC
publications citing this reagent: 10

quantity: 100 ug
price:
to the supplier

mouse monoclonal
reactivity: human, rat, mouse
application: WB, IHC, IC, IP, FC
publications citing this reagent: 14

quantity: 100 ug
price:
to the supplier

rabbit monoclonal
reactivity: human
conjugate: DyLight488
application: IC

quantity: 100 ul
price:
to the supplier

rabbit polyclonal
reactivity: human
modification: phosphorylated
application: WB

quantity: 100 µg
price: 270.00 USD
to the supplier

mouse polyclonal
reactivity: human
application: ELISA, WB

quantity: 50 uL
price: quote
to the supplier

mouse monoclonal
reactivity: human
application: ELISA

quantity: 100 ug
price: quote
to the supplier

mouse monoclonal
reactivity: human
application: WB, ELISA, IP

quantity: 100 uL
price: quote
to the supplier

rabbit monoclonal
reactivity: human
conjugate: DyLight488
application: IC

quantity: 100 ul
price:
to the supplier

mouse monoclonal
reactivity: human, mouse
application: WB, IHC, IC, IP, IHC-P

Human Kidney (formalin-fixed, paraffin-embedded) stained with ATM antibody LS-B1683 at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody LS-D2, alkaline phosphatase-streptavidin and chromogen.

Human Testis (formalin-fixed, paraffin-embedded) stained with ATM antibody LS-B1683 at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody LS-D2, alkaline phosphatase-streptavidin and chromogen.
quantity:
price:
to the supplier

mouse monoclonal
reactivity: human
application: WB, IHC, IP, IHC-P

Human Kidney (formalin-fixed, paraffin-embedded) stained with ATM antibody LS-B1684 at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody LS-D2, alkaline phosphatase-streptavidin and chromogen.
quantity:
price:
to the supplier

rabbit polyclonal
reactivity: human
application: WB, IHC, IHC-P

quantity:
price:
to the supplier

mouse monoclonal
reactivity: human, rat, mouse
application: WB, IC, IP
publications citing this reagent: 7

quantity: 100 µg/ml
price: 303 USD
to the supplier

mouse monoclonal
reactivity: human, rat, mouse
application: WB, IC, IP
publications citing this reagent: 4

quantity: 100 µg/ml
price: 295 USD
to the supplier

mouse monoclonal
reactivity: human, mouse
modification: phosphorylated
application: WB, IC, IP
publications citing this reagent: 6

quantity: 200 µg/ml
price: 279 USD
to the supplier

mouse monoclonal
reactivity: human

quantity: 200 ul
price: 406.50 USD
to the supplier

mouse monoclonal
reactivity: human
modification: phosphorylated

quantity: 200 ug
price: 398.65 USD
to the supplier

rabbit polyclonal
reactivity: human
modification: phosphorylated
application: IC

quantity: 50 ug
price: 409.04 USD
to the supplier

rabbit polyclonal
reactivity: human
application: WB, IHC, IC, IP
publications citing this reagent: 54

Western Blot: ATM Antibody [NB100-104] - Detection of ATM in HeLa nuclear extract using NB100-104. (30 sec. exposure)

Immunohistochemistry: ATM Antibody [NB100-104] - Staining of Testis, Seminiferous Tubule using NB100-104.
quantity: 0.05 ml
price: 295.00 USD
to the supplier

mouse monoclonal
reactivity: human, rat, mouse
application: WB, IHC, IC, IP
publications citing this reagent: 8

Western Blot: ATM Kinase Phosphospecific Antibody (10H11.E12) [NB100-306] - Western Blot analysis of ATM-kinase, using NB100-306. Sample: Irradiated or peroxidated human fibroblasts.
quantity: 0.1 ml
price: 295.00 USD
to the supplier

mouse monoclonal
reactivity: human, mouse
application: WB
publications citing this reagent: 2

Western Blot: ATM Kinase [Ser 1981][NB100-307]: Western Blot on HeLa crude extract, 5 Gy ionizing radiation.
quantity: 0.1 ml
price: 295.00 USD
to the supplier