rabbit polyclonal
reactivity: human, rat, cow, mouse
application: WB, ELISA, IHC, IP
publications citing this reagent: 1

Western Blot of Rabbit anti-Collagen I antibody. Lane 1: Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced). Lane 2: PPARg-transduced cell lysates. Load: 100 ug per lane. Protein staining shown below each blot depicts equal protein loading. Primary antibody: anti-Collagen I antibody at 0.2–2 ug/10 ml for overnight at 4°C. Secondary antibody: horseradish peroxidase-conjugated rabbit secondary antibody at 1 ug/10 ml for overnight at 4°C. Block: TBS with 5% Non-fat milk. Predicted/Observed size: 138.9 kDa for Collagen I. Other band(s): none.
quantity: 100 ug
price: 289.00 USD
to the supplier

rabbit polyclonal
reactivity: human, rat, cow, mouse
application: WB, ELISA, IHC, IP

Immunohistochemistry of Collagen I antibody. Tissue: human lung. Fixation: formalin fixed paraffin embedded. Antigen retrieval: user optimized. Primary antibody: Collagen I 1:400. Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT. Localization: Strong staining was observed in the extracellular matrix of the lung. Epithelial cells were negative. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.

Rockland's Affinity Purified anti-Collagen I antibody was used at a 1:100 dilution to detect distal tubules in normal kidney tissue. Note the absence of staining of glomeruli. The antibody was reacted with antibody for 4 hours at room temperature followed by the addition of secondary antibody and substrate reaction. Tissue was formalin-fixed and paraffin embedded. No antigen retrieval was performed.

Western blot analysis is shown using Rockland's Affinity Purified anti-Collagen I antibody to detect expression of collagen I in Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced) (left lane) and PPARg-transduced cell lysates (right lane). Protein staining shown below each blot depicts equal protein loading.  An equal amount of the whole cell protein (100 ug) was separated by SDS-PAGE and electroblotted to nitro-cellulose membranes.  Proteins were detected by incubating the membrane with anti-Collagen I antibody at a concentration of 0.2–2 ug/10 ml in TBS (100 mM Tris-HCl, 0.15 M NaCl, pH 7.4) with 5% Non-fat milk.  Detection occurred by incubation with a horseradish peroxidase-conjugated secondary antibody at 1 ug/10 ml.  Proteins were detected by a chemiluminescent method using the PIERCE ECL kit (Amersham Biosciences).  Other detection systems will yield similar results. See Hazra et al. (2004) for additional details.
quantity: 100 ug
price: 289.00 USD
to the supplier

rabbit polyclonal
reactivity: human, rat, cow, mouse
application: WB, ELISA, IHC, IP

Immunohistochemistry of Collagen I antibody. Tissue: Normal Kidney. Fixation: formalin fixed paraffin embedded. Antigen retrieval: No antigen retrieval was performed. Primary antibody: Collagen I 1:100 4 hours at room temperature Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT. Localization: Distal tubules in normal kidney tissue. Note the absence of staining of glomeruli. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.

Immunohistochemistry of Collagen I antibody. Tissue: Normal Kidney. Fixation: formalin fixed paraffin embedded. Antigen retrieval: No antigen retrieval was performed. Primary antibody: Collagen I 1:100 4 hours at room temperature Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT. Localization: Distal tubules in normal kidney tissue. Note the absence of staining of glomeruli. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.

Western blot analysis is shown using Rockland's Affinity Purified anti-Collagen I antibody to detect expression of collagen I in Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced) (left lane) and PPARg-transduced cell lysates (right lane). Protein staining shown below each blot depicts equal protein loading. An equal amount of the whole cell protein (100 ug) was separated by SDS-PAGE and electroblotted to nitro-cellulose membranes. Proteins were detected by incubating the membrane with anti-Collagen I antibody at a concentration of 0.2–2 ug/10 ml in TBS (100 mM Tris-HCl, 0.15 M NaCl, pH 7.4) with 5% Non-fat milk. Detection occurred by incubation with a horseradish peroxidase-conjugated secondary antibody at 1 ug/10 ml. Proteins were detected by a chemiluminescent method using the PIERCE ECL kit (Amersham Biosciences). Other detection systems will yield similar results. See Hazra et al. (2004) for additional details.
quantity: 500 ug
price: 575.00 USD
to the supplier

mouse monoclonal
reactivity: human, rat, cow
application: ELISA, IHC, IC, FC, IHC-F
publications citing this reagent: 2

quantity: 100 µl
price: 364.00 USD
to the supplier

rabbit polyclonal
reactivity: human, cow
application: WB, ELISA, IHC, IP, FC, IHC-F

quantity: 50 µg
price: 325.00 USD
to the supplier

rabbit polyclonal
reactivity: cow, dog
application: ELISA, IHC, FC, IHC-F

quantity: 100 µg
price: 324.00 USD
to the supplier

mouse monoclonal
reactivity: human, rat, cow
application: ELISA, IHC, IC
publications citing this reagent: 1

quantity: 100 ul
price: 269 USD
to the supplier

rabbit polyclonal
reactivity: human, cow
application: WB, ELISA, IHC, IP

quantity: 50 ug
price: 269 USD
to the supplier

rabbit polyclonal
reactivity: human, rat, cow, dog, mouse

quantity: 100 ug
price: 296 USD
to the supplier

rabbit polyclonal
reactivity: human, rat, cow, dog, mouse

quantity: 100 ug
price: 296 USD
to the supplier

rabbit polyclonal
reactivity: human, rat, cow, dog, mouse
application: WB, ELISA, IC, IP, IHC-P, IHC-F
publications citing this reagent: 1

quantity: 100 ul or 100 ug lyo
price: 220 USD
to the supplier

mouse monoclonal
reactivity: human, rat, cow
application: WB, ELISA, IHC, IC, IHC-P
publications citing this reagent: 1

quantity: 100 µl ascites
price: 279 USD
to the supplier

rabbit polyclonal
reactivity: human, rat, cow, mouse
application: WB, IHC, IP

quantity: 500 µg/ml, 25% ag
price: 366 USD
to the supplier

rabbit polyclonal
reactivity: cow

quantity: 1 ml
price: 495
to the supplier

rabbit polyclonal
reactivity: cow
application: WB, ELISA, IHC, IC, RIA, IHC-P

quantity: 100 uL
price: quote
to the supplier

rabbit polyclonal
reactivity: cow
application: ELISA, IHC, IC, IHC-F

quantity: 1 mL
price: quote
to the supplier